| 质粒名称: | jk100 pSinEGdsp(p1:MAPPnL;p2:gfp:barcodelandingpad:4xboxB) |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 79785 |
| 存储实验室: | Anthony Zador |
|
载体骨架基本信息 |
|
| 载体名称: | pSinEGdsp |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | 13220 bp |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达，合成生物学；辛德比斯病毒基因组质粒 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | -- |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | MAPP-nL |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | jk100 pSinEGdsp(p1:MAPPnL;p2:gfp:barcodelandingpad:4xboxB) was a gift from Anthony Zador (Addgene plasmid # 79785 ; http://n2t.net/addgene:79785 ; RRID:Addgene_79785) |
jk100 pSinEGdsp(p1:MAPPnL;p2:gfp:barcodelandingpad:4xboxB)
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
质粒图谱.png)
载体简介
载体序列
LOCUS jk100 pSinEGdsp(p1:MAPPnL;p2:gfp:barcodelandingpad:4xboxB) 13220 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION genomic Sindbis plasmid, encoding MAPPnL and a GFP RNA with a
barcode landing pad.
ACCESSION .
VERSION .
KEYWORDS jk100 pSinEGdsp(p1:MAPPnL;p2:gfp:barcodelandingpad:4xboxB)
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 13220)
TITLE Zador Lab MAPseq plasmids
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 13220)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..13220
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS 7753..7800
/codon_start=1
/product="11th beta-strand of superfolder GFP"
/label=GFP11
/note="associates with GFP(1-10), consisting of the first
10 beta-strands, to reconstitute fluorescent GFP (Cabantous
et al., 2005; Kamiyama et al., 2016)"
/translation="RDHMVLHEYVNAAGIT"
CDS 7837..7866
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
CDS 7873..8418
/codon_start=1
/product="human O6-alkylguanine-DNA-alkyltransferase
variant that forms covalent bonds with benzylcytosine
derivatives"
/label=CLIP-tag(TM)
/note="optimized for mammalian expression"
/translation="MDKDCEMKRTTLDSPLGKLELSGCEQGLHRIIFLGKGTSAADAVE
VPAPAAVLGGPEPLIQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLK
VVKFGEVISESHLAALVGNPAATAAVNTALDGNPVPILIPCHRVVQGDSDVGPYLGGLA
VKEWLLAHEGHRLGKPGLG"
regulatory 9187..9196
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
regulatory 9316..9325
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
regulatory 9445..9454
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
regulatory 9574..9583
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
regulatory 9976..9985
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 9982..10701
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(10027..10048)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(10288..10307)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 10635..10656
/label=EGFP-C
/note="EGFP, forward primer"
promoter 11391..11495
/gene="bla"
/label=AmpR promoter
CDS 11496..12356
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(11714..11733)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 12527..13115
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 13016..13035
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 attgacggcg tagtacacac tattgaatca aacagccgac caattgcact accatcacaa
61 tggagaagcc agtagtaaac gtagacgtag acccccagag tccgtttgtc gtgcaactgc
121 aaaaaagctt cccgcaattt gaggtagtag cacagcaggt cactccaaat gaccatgcta
181 atgccagagc attttcgcat ctggccagta aactaatcga gctggaggtt cctaccacag
241 cgacgatctt ggacataggc agcgcaccgg ctcgtagaat gttttccgag caccagtatc
301 attgtgtctg ccccatgcgt agtccagaag acccggaccg catgatgaaa tacgccagta
361 aactggcgga aaaagcgtgc aagattacaa acaagaactt gcatgagaag attaaggatc
421 tccggaccgt acttgatacg ccggatgctg aaacaccatc gctctgcttt cacaacgatg
481 ttacctgcaa catgcgtgcc gaatattccg tcatgcagga cgtgtatatc aacgctcccg
541 gaactatcta tcatcaggct atgaaaggcg tgcggaccct gtactggatt ggcttcgaca
601 ccacccagtt catgttctcg gctatggcag gttcgtaccc tgcgtacaac accaactggg
661 ccgacgagaa agtccttgaa gcgcgtaaca tcggactttg cagcacaaag ctgagtgaag
721 gtaggacagg aaaattgtcg ataatgagga agaaggagtt gaagcccggg tcgcgggttt
781 atttctccgt aggatcgaca ctttatccag aacacagagc cagcttgcag agctggcatc
841 ttccatcggt gttccacttg aatggaaagc agtcgtacac ttgccgctgt gatacagtgg
901 tgagttgcga aggctacgta gtgaagaaaa tcaccatcag tcccgggatc acgggagaaa
961 ccgtgggata cgcggttaca cacaatagcg agggcttctt gctatgcaaa gttactgaca
1021 cagtaaaagg agaacgggta tcgttccctg tgtgcacgta catcccggcc accatatgcg
1081 atcagatgac tggtataatg gccacggata tatcacctga cgatgcacaa aaacttctgg
1141 ttgggctcaa ccagcgaatt gtcattaacg gtaggactaa caggaacacc aacaccatgc
1201 aaaattacct tctgccgatc atagcacaag ggttcagcaa atgggctaag gagcgcaagg
1261 atgatcttga taacgagaaa atgctgggta ctagagaacg caagcttacg tatggctgct
1321 tgtgggcgtt tcgcactaag aaagtacatt cgttttatcg cccacctgga acgcagacct
1381 gcgtaaaagt cccagcctct tttagcgctt ttcccatgtc gtccgtatgg acgacctctt
1441 tgcccatgtc gctgaggcag aaattgaaac tggcattgca accaaagaag gaggaaaaac
1501 tgctgcaggt ctcggaggaa ttagtcatgg aggccaaggc tgcttttgag gatgctcagg
1561 aggaagccag agcggagaag ctccgagaag cacttccacc attagtggca gacaaaggca
1621 tcgaggcagc cgcagaagtt gtctgcgaag tggaggggct ccaggcggac atcggagcag
1681 cattagttga aaccccgcgc ggtcacgtaa ggataatacc tcaagcaaat gaccgtatga
1741 tcggacagta tatcgttgtc tcgccaaact ctgtgctgaa gaatgccaaa ctcgcaccag
1801 cgcacccgct agcagatcag gttaagatca taacacactc cggaagatca ggaaggtacg
1861 cggtcgaacc atacgacgct aaagtactga tgccagcagg aggtgccgta ccatggccag
1921 aattcctagc actgagtgag agcgccacgt tagtgtacaa cgaaagagag tttgtgaacc
1981 gcaaactata ccacattgcc atgcatggcc ccgccaagaa tacagaagag gagcagtaca
2041 aggttacaaa ggcagagctt gcagaaacag agtacgtgtt tgacgtggac aagaagcgtt
2101 gcgttaagaa ggaagaagcc tcaggtctgg tcctctcggg agaactgacc aaccctccct
2161 atcatgagct agctctggag ggactgaaga cccgacctgc ggtcccgtac aaggtcgaaa
2221 caataggagt gataggcaca ccggggtcgg gcaagtcagc tattatcaag tcaactgtca
2281 cggcacgaga tcttgttacc agcggaaaga aagaaaattg tcgcgaaatt gaggccgacg
2341 tgctaagact gaggggtatg cagattacgt cgaagacagt agattcggtt atgctcaacg
2401 gatgccacaa agccgtagaa gtgctgtacg ttgacgaagc gttcgcgtgc cacgcaggag
2461 cactacttgc cttgattgct atcgtcaggc cccgcaagaa ggtagtacta tgcggagacc
2521 ccatgcaatg cggattcttc aacatgatgc aactaaaggt acatttcaat caccctgaaa
2581 aagacatatg caccaagaca ttctacaagt atatctcccg gcgttgcaca cagccagtta
2641 cagctattgt atcgacactg cattacgatg gaaagatgaa aaccacgaac ccgtgcaaga
2701 agaacattga aatcgatatt acaggggcca caaagccgaa gccaggggat atcatcctga
2761 catgtttccg cgggtgggtt aagcaattgc aaatcgacta tcccggacat gaagtaatga
2821 cagccgcggc ctcacaaggg ctaaccagaa aaggagtgta tgccgtccgg caaaaagtca
2881 atgaaaaccc actgtacgcg atcacatcag agcatgtgaa cgtgttgctc acccgcactg
2941 aggacaggct agtgtggaaa accttgcagg gcgacccatg gattaagcag cccactaaca
3001 tacctaaagg aaactttcag gctactatag aggactggga agctgaacac aagggaataa
3061 ttgctgcaat aaacagcccc actccccgtg ccaatccgtt cagctgcaag accaacgttt
3121 gctgggcgaa agcattggaa ccgatactag ccacggccgg tatcgtactt accggttgcc
3181 agtggagcga actgttccca cagtttgcgg atgacaaacc acattcggcc atttacgcct
3241 tagacgtaat ttgcattaag tttttcggca tggacttgac aagcggactg ttttctaaac
3301 agagcatccc actaacgtac catcccgccg attcagcgag gccggtagct cattgggaca
3361 acagcccagg aacccgcaag tatgggtacg atcacgccat tgccgccgaa ctctcccgta
3421 gatttccggt gttccagcta gctgggaagg gcacacaact tgatttgcag acggggagaa
3481 ccagagttat ctctgcacag cataacctgg tcccggtgaa ccgcaatctt cctcacgcct
3541 tagtccccga gtacaaggag aagcaacccg gcccggtcaa aaaattcttg aaccagttca
3601 aacaccactc agtacttgtg gtatcagagg aaaaaattga agctccccgt aagagaatcg
3661 aatggatcgc cccgattggc atagccggtg cagataagaa ctacaacctg gctttcgggt
3721 ttccgccgca ggcacggtac gacctggtgt tcatcaacat tggaactaaa tacagaaacc
3781 accactttca gcagtgcgaa gaccatgcgg cgaccttaaa aaccctttcg cgttcggccc
3841 tgaattgcct taacccagga ggcaccctcg tggtgaagtc ctatggctac gccgaccgca
3901 acagtgagga cgtagtcacc gctcttgcca gaaagtttgt cagggtgtct gcagcgagac
3961 cagattgtgt ctcaagcaat acagaaatgt acctgatttt ccgacaacta gacaacagcc
4021 gtacacggca attcaccccg caccatctga attgcgtgat ttcgtccgtg tatgagggta
4081 caagagatgg agttggagcc gcgccgtcat accgcaccaa aagggagaat attgctgact
4141 gtcaagagga agcagttgtc aacgcagcca atccgctggg tagaccaggc gaaggagtct
4201 gccgtgccat ctataaacgt tggccgacca gttttaccga ttcagccacg gagacaggca
4261 ccgcaagaat gactgtgtgc ctaggaaaga aagtgatcca cgcggtcggc cctgatttcc
4321 ggaagcaccc agaagcagaa gccttgaaat tgctacaaaa cgcctaccat gcagtggcag
4381 acttagtaaa tgaacataac atcaagtctg tcgccattcc actgctatct acaggcattt
4441 acgcagccgg aaaagaccgc cttgaagtat cacttaactg cttgacaacc gcgctagaca
4501 gaactgacgc ggacgtaacc atctattgcc tggataagaa gtggaaggaa agaatcgacg
4561 cggcactcca acttaaggag tctgtaacag agctgaagga tgaagatatg gagatcgacg
4621 atgagttagt atggattcat ccagacagtt gcttgaaggg aagaaaggga ttcagtacta
4681 caaaaggaaa attgtattcg tacttcgaag gcaccaaatt ccatcaagca gcaaaagaca
4741 tggcggagat aaaggtcctg ttccctaatg accaggaaag taatgaacaa ctgtgtgcct
4801 acatattggg tgagaccatg gaagcaatcc gcgaaaagtg cccggtcgac cataacccgt
4861 cgtctagccc gcccaaaacg ttgccgtgcc tttgcatgta tgccatgacg ccagaaaggg
4921 tccacagact tagaagcaat aacgtcaaag aagttacagt atgctcctcc accccccttc
4981 ctaagcacaa aattaagaat gttcagaagg ttcagtgcac gaaagtagtc ctgtttaatc
5041 cgcacactcc cgcattcgtt cccgcccgta agtacataga agtgccagaa cagcctaccg
5101 ctcctcctgc acaggccgag gaggcccccg aagttgtagc gacaccgtca ccatctacag
5161 ctgataacac ctcgcttgat gtcacagaca tctcactgga tatggatgac agtagcgaag
5221 gctcactttt ttcgagcttt agcggatcgg acaactctat tactagtatg gacagttggt
5281 cgtcaggacc tagttcacta gagatagtag accgaaggca ggtggtggtg gctgacgttc
5341 atgccgtcca agagcctgcc cctattccac cgccaaggct aaagaagatg gcccgcctgg
5401 cagcggcaag aaaagagccc actccaccgg caagcaatag ctctgagtcc ctccacctct
5461 cttttggtgg ggtatccatg tccctcggat caattttcga cggagagacg gcccgccagg
5521 cagcggtaca acccctggca acaggcccca cggatgtgcc tatgtctttc ggatcgtttt
5581 ccgacggaga gattgatgag ctgagccgca gagtaactga gtccgaaccc gtcctgtttg
5641 gatcatttga accgggcgaa gtgaactcaa ttatatcgtc ccgatcagcc gtatcttttc
5701 cactacgcaa gcagagacgt agacgcagga gcaggaggac tgaatactga ctaaccgggg
5761 taggtgggta catattttcg acggacacag gccctgggca cttgcaaaag aagtccgttc
5821 tgcagaacca gcttacagaa ccgaccttgg agcgcaatgt cctggaaaga attcatgccc
5881 cggtgctcga cacgtcgaaa gaggaacaac tcaaactcag gtaccagatg atgcccaccg
5941 aagccaacaa aagtaggtac cagtctcgta aagtagaaaa tcagaaagcc ataaccactg
6001 agcgactact gtcaggacta cgactgtata actctgccac agatcagcca gaatgctata
6061 agatcaccta tccgaaacca ttgtactcca gtagcgtacc ggcgaactac tccgatccac
6121 agttcgctgt agctgtctgt aacaactatc tgcatgagaa ctatccgaca gtagcatctt
6181 atcagattac tgacgagtac gatgcttact tggatatggt agacgggaca gtcgcctgcc
6241 tggatactgc aaccttctgc cccgctaagc ttagaagtta cccgaaaaaa catgagtata
6301 gagccccgaa tatccgcagt gcggttccat cagcgatgca gaacacgcta caaaatgtgc
6361 tcattgccgc aactaaaaga aattgcaacg tcacgcagat gcgtgaactg ccaacactgg
6421 actcagcgac attcaatgtc gaatgctttc gaaaatatgc atgtaatgac gagtattggg
6481 aggagttcgc tcggaagcca attaggatta ccactgagtt tgtcaccgca tatgtagcta
6541 gactgaaagg ccctaaggcc gccgcactat ttgcaaagac gtataatttg gtcccattgc
6601 aagaagtgcc tatggataga ttcgtcatgg acatgaaaag agacgtgaaa gttacaccag
6661 gcacgaaaca cacagaagaa agaccgaaag tacaagtgat acaagccgca gaacccctgg
6721 cgactgctta cttatgcggg attcaccggg aattagtgcg taggcttacg gccgtcttgc
6781 ttccaaacat tcacacgctt tttgacatgt cggcggagga ttttgatgca atcatagcag
6841 aacacttcaa gcaaggcgac ccggtactgg agacggatat cgcatcattc gacaaaagcc
6901 aagacgacgc tatggcgtta accggtctga tgatcttgga ggacctgggt gtggatcaac
6961 cactactcga cttgatcgag tgcgcctttg gagaaatatc atccacccat ctacctacgg
7021 gtactcgttt taaattcggg gcgatgatga aatccggaat gttcctcaca ctttttgtca
7081 acacagtttt gaatgtcgtt atcgccagca gagtactaga agagcggctt aaaacgtcca
7141 gatgtgcagc gttcattggc gacgacaaca tcatacatgg agtagtatct gacaaagaaa
7201 tggctgagag gtgcgccacc tggctcaaca tggaggttaa gatcatcgac gcagtcatcg
7261 gtgagagacc accttacttc tgcggcggat ttatcttgca agattcggtt acttccacag
7321 cgtgccgcgt ggcggatccc ctgaaaaggc tgtttaagtt gggtaaaccg ctcccagccg
7381 acgacgagca agacgaagac agaagacgcg ctctgctaga tgaaacaaag gcgtggttta
7441 gagtaggtat aacaggcact ttagcagtgg ccgtgacgac ccggtatgag gtagacaata
7501 ttacacctgt cctactggca ttgagaactt ttgcccagag caaaagagca ttccaagcca
7561 tcagagggga aattaaagca tctctacggt ggtcctaaat agtcagcata gtacatttca
7621 tctgactaat accacaacac caccacctct agagccacca tgccaccttc tactagtctt
7681 ttgttgcttg ctgcacttct ccccttcgct cttcccgctt cagattggaa gacaggagaa
7741 gtcaccgctt cccgcgacca catggtgctg cacgaatatg tgaacgctgc tggcatcaca
7801 ggcggtggcg gatctggcgg cggtggtagc gtcgacgaac aaaaactcat ctcagaagag
7861 gatctacaat tcatggacaa agactgcgaa atgaagcgca ccaccctgga tagccctctg
7921 ggcaagctgg aactgtctgg gtgcgaacag ggcctgcacc gtatcatctt cctgggcaaa
7981 ggaacatctg ccgccgacgc cgtggaagtg cctgccccag ccgccgtgct gggcggacca
8041 gagccactga tccaggccac cgcctggctc aacgcctact ttcaccagcc tgaggccatc
8101 gaggagttcc ctgtgccagc cctgcaccac ccagtgttcc agcaggagag ctttacccgc
8161 caggtgctgt ggaaactgct gaaagtggtg aagttcggag aggtcatcag cgagagccac
8221 ctggccgccc tggtgggcaa tcccgccgcc accgccgccg tgaacaccgc cctggacgga
8281 aatcccgtgc ccattctgat cccctgccac cgggtggtgc agggcgacag cgacgtgggg
8341 ccctacctgg gcgggctcgc cgtgaaagag tggctgctgg cccacgaggg ccacagactg
8401 ggcaagcctg ggctgggtgg cggtggcgga tctgtcgact ttcagaaagc tagctctatt
8461 gtttataaga aggaggggga acaggtggag ttcagtttcc cattggcctt cacagtcgag
8521 aagctcacag gatcaggaga gttgtggtgg caggccgaaa gagcctcctc cagcaagagt
8581 tggatcactt ttgatctgaa aaacaaagaa gtgagcgtga agagagtcac acaggaccct
8641 aagctgcaga tgggcaagaa gttgcctttg cacttgacac tcccccaagc cctgccccag
8701 tatgctggct ccgggaacct caccctcgcc ttggaggcta agaccggaaa gttgcatcag
8761 gaggtcaacc tcgtggtgat gagggcaaca cagctgcaga agaacctgac ctgcgaagtg
8821 tggggtccca cctcacctaa actgatgctg tctctgaagc tggagaataa ggaggctaaa
8881 gtcagtaaaa gagagaaggc tgtgtgggtc cttaacccgg aggcagggat gtggcagtgt
8941 cttcttagcg actctggaca ggtgctgctg gagagcaaca tcaaggtgct tcctacttgg
9001 tcaacaccag tacagccgat ggccctgatc gtactcgggg gagtcgccgg cctgcttctg
9061 tttatcggcc tgggcatctt cttctgcgta cgctacaagt atcgcaacag ggatgaggga
9121 tctagcgctc caccgctcga cggagccgga gctggcgctg gagctggagc cggagctggc
9181 gggctagcca ccatggacgc acaaacacga cgacgtgagc gtcgcgctga gaaacaagct
9241 caatggaaag ctgcaaaccc accgctcgac ggagccggag ctggcgctgg agctggagcc
9301 ggagctggcg gtctagccac catggacgca caaacacgac gacgtgagcg tcgcgctgag
9361 aaacaagctc aatggaaagc tgcaaaccca ccgctcgacg gagccggagc tggcgctgga
9421 gctggagccg gagctggcgg tctagccacc atggacgcac aaacacgacg acgtgagcgt
9481 cgcgctgaga aacaagctca atggaaagct gcaaacccac cgctcgacgg agccggagct
9541 ggcgctggag ctggagccgg agctggcggt ctagccacca tggacgcaca aacacgacga
9601 cgtgagcgtc gcgctgagaa acaagctcaa tggaaagctg caaacccacc gctcgagcca
9661 ccgctcgacg gagccggagc tggcgctgga gctggagccg gagctggcgg tctagccacc
9721 ccaccgagcg cttaccacgt tgacgaatct aggaactata tcagtaacag tgctcagagt
9781 aatggcgccg tggttaaaga gaaacagccc tccagcgcca aatcagcaaa caaaaacaag
9841 aaaaacaagg ataaagagta ctacgtatag gcggcgcatg caggccttaa agcatctcta
9901 cggtggtcct aaatagtcag catagtacat ttcatctgac taataccaca acaccaccac
9961 ctctagtaga ggcgcgccac catggtgagc aagggcgagg agctgttcac cggggtggtg
10021 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
10081 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
10141 ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc
10201 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
10261 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
10321 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
10381 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc
10441 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag
10501 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
10561 gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac
10621 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
10681 atggacgagc tgtacaagta aacgcgtgag aacttcagcg gccgctacct aattgccgtc
10741 gtgaggtacg accaccgcta gctgtacagc cctgaaaaag ggctcgagcc ctgaaaaagg
10801 gcaattgccc tgaaaaaggg cgtcgacgcc ctgaaaaagg gcggaattca tgtccaattt
10861 actgactgta caccaaaatt tgcctgcggg cccaatgatc cgaccagcaa aactcgatgt
10921 acttccgagg aactgatgtg cataatgcat caggctggta cattagatcc ccgcttaccg
10981 cgggcaatat agcaacacta aaaactcgat gtacttccga ggaagcgcag tgcataatgt
11041 tgcgcagtgt tgccacataa ccactatatt aaccatttat ttagcggacg ccaaaaactc
11101 aatgtatttt tgaggaagcg tggtgcataa tgccacgcag gttttgcata attttttttt
11161 ttttttttat taatcaacaa aattttgttt ttaacatttc aaaaaaaaaa aaaaaaaaaa
11221 aaaaaaaaaa aaaaggggaa ttcctcgata attaagcggc ctagattatg taggttgtga
11281 gatgcgggag gttctcgatc ttcccgtggg acgtcaaggc cgcttaatta atcgagggga
11341 attaattctt gaagacgaaa gggccaggtg gcacttttcg gggaaatgtg cgcggaaccc
11401 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
11461 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
11521 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
11581 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
11641 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
11701 cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac
11761 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
11821 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
11881 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
11941 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
12001 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
12061 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
12121 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
12181 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
12241 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
12301 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
12361 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
12421 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
12481 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
12541 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
12601 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
12661 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
12721 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
12781 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
12841 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
12901 gatacctaca gcgtgagcat tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
12961 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
13021 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
13081 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg agctcgtatg
13141 gacatattgt cgttagaacg cggctacaat taatacataa ccttatgtat catacacata
13201 cgatttaggg gacactatag
//
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